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diffuse large b cell lymphoma cell lines sudhl4  (ATCC)


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    ATCC diffuse large b cell lymphoma cell lines sudhl4
    Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines <t>(SUDHL4</t> and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.
    Diffuse Large B Cell Lymphoma Cell Lines Sudhl4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffuse large b cell lymphoma cell lines sudhl4/product/ATCC
    Average 96 stars, based on 431 article reviews
    diffuse large b cell lymphoma cell lines sudhl4 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Tumor‐Associated Sympathetic Nerves Promote the Progression of Epstein‐Barr Virus‐Positive Diffuse Large B‐Cell Lymphoma"

    Article Title: Tumor‐Associated Sympathetic Nerves Promote the Progression of Epstein‐Barr Virus‐Positive Diffuse Large B‐Cell Lymphoma

    Journal: Advanced Science

    doi: 10.1002/advs.202413580

    Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines (SUDHL4 and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.
    Figure Legend Snippet: Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines (SUDHL4 and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.

    Techniques Used: Virus, Infection, Derivative Assay, Gene Expression, Reverse Transcription, Quantitative RT-PCR, Western Blot, Expressing



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    96
    ATCC diffuse large b cell lymphoma cell lines sudhl4
    Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines <t>(SUDHL4</t> and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.
    Diffuse Large B Cell Lymphoma Cell Lines Sudhl4, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffuse large b cell lymphoma cell lines sudhl4/product/ATCC
    Average 96 stars, based on 1 article reviews
    diffuse large b cell lymphoma cell lines sudhl4 - by Bioz Stars, 2026-03
    96/100 stars
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    Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines (SUDHL4 and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.

    Journal: Advanced Science

    Article Title: Tumor‐Associated Sympathetic Nerves Promote the Progression of Epstein‐Barr Virus‐Positive Diffuse Large B‐Cell Lymphoma

    doi: 10.1002/advs.202413580

    Figure Lengend Snippet: Establishment of Epstein‐Barr virus (EBV)‐infected diffuse large B‐cell lymphoma (DLBCL) cell lines. A) Schematic illustration showing the process of establishing EBV‐positive DLBCL cell lines. EBV‐negative DLBCL cell lines (SUDHL4 and SUDHL6) were infected with cell‐free EBV derived from Akata cells and subsequently selected using G418. This figure was created by Figdraw under copyright code‐[RURPI77371]. B) Quantification of latency gene expression in different cell lines by quantitative reverse transcription PCR (qRT‐PCR), including EBER1, EBER2, LMP1, LMP2A, EBNA1, EBNA2, EBNA3A, EBNA3B, and EBNA3C. Gene expression was normalized to β‐actin. C) Western blot analysis confirms the expression of EBNA2 and LMP1 in the indicated cell lines. Akata cells were stimulated with 0.8% goat anti‐human IgG for 6 h. D) Mapping analysis of transcript sequences from tumors derived from SUDHL6‐EBV or SUDHL6 cells against the Akata genome. E) Assessment of latency genes expression in xenograft tumors from EBV positive DLBCL cells and their parental counterparts. F) Representative images of EBERs‐ISH in tumor tissues from mice implanted with EBV‐positive DLBCL cells or their parental cells.

    Article Snippet: The human diffuse large B‐cell lymphoma cell lines SUDHL4, SUDHL6, and Farage were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Virus, Infection, Derivative Assay, Gene Expression, Reverse Transcription, Quantitative RT-PCR, Western Blot, Expressing